Cholinergic Synaptic Vesicles from Torpedo
نویسندگان
چکیده
Whittaker, V. P. (1971a) Ann. N. Y. Acad. Sci. 183, 21 Whittaker, V. P. (1971b) Advan. Cytopharmacol. 1, 319 Whittaker, V. P. (1972) in Structure and Function of Synapses (Pappas, G. D. & Purpura, D. P., eds.), Raven Press, New York Whittaker, V. P. & Barker, L. A. (1971) Methods Neurochem. 2, 1 Whittaker, V. P. & Sheridan, M. N. (1965) J. Neurochem. 12, 363 Whittaker, V. P., Michaelson, I. A. & Kirkland, R. J. A. (1964) Biochem. J. 90, 293
منابع مشابه
Individual synaptic vesicles from the electroplaque of Torpedo californica, a classic cholinergic synapse, also contain transporters for glutamate and ATP
The type of neurotransmitter secreted by a neuron is a product of the vesicular transporters present on its synaptic vesicle membranes and the available transmitters in the local cytosolic environment where the synaptic vesicles reside. Synaptic vesicles isolated from electroplaques of the marine ray, Torpedo californica, have served as model vesicles for cholinergic neurotransmission. Many lin...
متن کاملCholinergic nerve terminals contain ascorbic acid which induces Ca2+-dependent release of acetylcholine and ATP from isolated Torpedo synaptic vesicles.
We have shown that a soluble, low-molecular weight, alkali-labile and oxidation-sensitive factor, prepared from the electric organ of Torpedo ocellata, induces Cap-dependent acetylcholine (ACh) release from isolated Torpedo synaptic vesicles [ 1,2]. Diverse activities of ascorbic acid in the brain have been reported [3-81. These include effects on metabolism of biogenic amines [9] and on releas...
متن کاملSynapsin I is associated with cholinergic nerve terminals in the electric organs of Torpedo, Electrophorus, and Malapterurus and copurifies with Torpedo synaptic vesicles.
Using an affinity-purified monospecific polyclonal antibody against bovine brain synapsin I, the distribution of antigenically related proteins was investigated in the electric organs of the three strongly electric fish Torpedo marmorata, Electrophorus electricus, Malapterurus electricus and in the rat diaphragm. On application of indirect fluorescein isothiocyanate-immunofluorescence and using...
متن کاملIon channels in synaptic vesicles from Torpedo electric organ.
A simple method has been developed for fusing synaptic vesicles into spherical structures 20-50 micron in diameter. The method has been applied to purified cholinergic synaptic vesicles from Torpedo electric organ, and the membrane properties of these fused structures have been studied by the "cell"-attached version of the patch clamp technique. A large conductance potassium-preferring channel,...
متن کاملAsymmetry of lipid organization in cholinergic synaptic vesicle membranes.
The lipid composition of purified Torpedo cholinergic synaptic vesicles was determined and their distribution between the inner and outer leaflets of the vesicular membrane was investigated. The vesicles contain cholesterol and phospholipids at a molar ratio of 0.63. The vesicular phospholipids are (mol% of total phospholipids): phosphatidylcholine (40.9); phosphatidylethanolamine (24.6); plasm...
متن کامل